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In order to induce the microspore derived callus, embryos and plantlets, anthers of Zantedeschia aethiopica were cultured on Murashige and Skoog´s medium supplemented with various combinations of auxins and cytokinins. The most suitable stage of anther culture on the induction of callus and embryos was at the late uninucleate and early binucleate stages (10 days before anthesis), and these microspores were developed into the callus or embryos by repeated divisions of the vegetative nuclei. The combinations of NAA and BA were more effective than those of 2,4-D and kinetin in the formation of callus and embryos. Regenerated plantlets through organogenesis were observed on medium with 1.0㎎·L^(-1) 2,4-D and kinetin respectively and those through embryogenesis were on medium with 0.5㎎·L^(-1) NAA and 1.0㎎·L^(-1) BA or 2.0㎎·L^(-1) NAA and BA respectively. Low temperature pretreatment for 6 days at 5 was more effective to induce callus, embryos and growth of regenerated plantlets than the treatment of 10℃ and control. Haploid plantlet produced from one of all anthers cultured, on MS basal medium supplemented with 1.0㎎·L^(-1) NAA and 0.5㎎·L^(-1) BA. Root tip squashes of the regenerated plantlet showed the haploid chromosome number.
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